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Light Sheet Microscopy

Ultra Thin Light Sheet

Cleared Tissue & Live Specimen

Beyond Light Sheet Imaging

Light Sheet Fluorescence Microscopy has revolutionized the way how optical imaging of biological specimens can be performed, by maintaining their physiological integrity and minimizing light-induced photo-damage and photo-toxicity. We design highly sophisticated light sheet microscopes that provide optimal image quality, unrivaled user experience and the necessary flexibility for scientific imaging instruments.

OPTIMAL IMAGE QUALITY

Optimal Image Quality

Ultra thin sectioning for sub-cellular resolution

Our smart light sheet illuminators provide ultra-thin optical sections over a large field of view. When combined with high NA objectives, optimized XYZ resolution can be achieved. Our light sheet microscopy systems meet resolution requirements from small to very large sample imaging.

Patented high illumination NA and focus sweeping result in no trade-off between resolution and field of view
Ultra thin light sheet and wide field of view

Patented high-illumination NA and focus sweeping eliminate trade-offs between resolution and field of view.

Conventional Light Sheet Fluorescent Microscopy​

In conventional LSFM, the thinnest part of the light sheet is limited to a small field of view, and a large field of view comes at the expense of axial resolution. Therefore, conventional LSFM systems are mostly designed for specific sample type and size, and offer little flexibility to accommodate different sample sizes.

Trade-off between resolution and the field of view
Thick light sheet and wide field of view
Trade-off between resolution and the field of view
Thin light sheet and small field of view

Trade-off between resolution and the field of view.

Ultra thin sectioning for sub-cellular resolution​

Whole mouse brain by PhaseView Alpha3 Light Sheet Microscope
Ultra thin light sheet over the entire field of view

Conventional Light Sheet Fluorescent Microscopy​

Whole mouse brain by PhaseView Alpha3 Light Sheet Microscope
High resolution in the central part of the image only

PEGASOS cleared whole mouse brain. Courtesy of Pr. Edna Hardeman and Bio-Imaging Facility (BMIF) of UNSW Sydney.

Optimal Image Quality

Maximized resolution across the whole field of view

Patented focus sweeping produces a constant beam thickness across the entire field of view, resulting in homogeneous PSFs. Imaging parameters are consistent for quantitative imaging and image processing tools such as deconvolution software.

Waist Sweeping On: Sharp Image all across the Field of View
Thin light sheet and wide field of view

Patented real time focus sweeping technology.

Conventional Light Sheet Fluorescent Microscopy​

Gaussian beams exhibit light sheet divergence: axial resolution is not constant across the field of view and PSFs are not uniform. As a result, quantitative image analysis or image deconvolution requires complex post-processing.

Waist Sweeping Off: Sharp Image over a Small Field of View
Thin light sheet and small field of view

Gaussian beam divergence.

Ultra thin sectioning for sub-cellular resolution​

Whole mouse brain by PhaseView Alpha3 Light Sheet Microscope
High resolution over the entire field of view

Conventional Light Sheet Fluorescent Microscopy​

Whole mouse brain by PhaseView Alpha3 Light Sheet Microscope
High resolution in the center of the image stack only

PEGASOS cleared whole mouse brain. Courtesy of Dr. Genaro Hernandez, University of Texas Southwestern, Dallas, USA

Optimal Image Quality

Maximum light coverage and intensity homogeneity

Simultaneous dual illumination with high lateral NA provides optimal sample penetration, even with thick samples, and efficient artifact removal when light is absorbed or scattered. This state-of-the-art technology enables true qualitative and quantitative imaging.

High NA On: High dynamic and homogeneous intensity images
High dynamic range and homogeneous intensity images

Patented Simultaneous dual illumination and high lateral NA illumination technologies.

Conventional Light Sheet Fluorescent Microscopy​

Lack of intensity homogeneity, shadow or stripe artifacts are common in light sheet microscopy despite more or less complex optical solutions to eliminate undesirable effects associated with light sheet fluorescence microscopy.

High NA Off: Unwanted artefacts and shadows
Thin light sheet and small field of view

Optical architecture results in insufficient light penetration and poor image quality due to unwanted artifacts and shadows.

Flexibility and Modularity

Fast and easy sample mounting solutions

All mounting accessories are conveniently adapted for all sample types and sizes. Sample integrity and physiological conditions are maintained during image acquisition. Mounting accessories and sample chamber are highly resistant to corrosive media and can be easily cleaned.

Mounting solutions for small and large samples
Unique set of accessories for fast and reproducible sample mounting

Small Sample Mounting

Large Sample Mounting

Flexibility and Modularity

Multi-sample acquisition

Light sheet microscopes are well suited for high throughput imaging experiments, offering an extended sample holder with clamps to accommodate multiple samples. Software resources handle the acquisition of individual stacks under the desired imaging conditions.

Unique set of accessories for fast and reproducible multi-sample mounting

Examples of sample mounting on sample holder.

Flexibility and Modularity

Configurable microscopy systems with a wide range of illumination and detection lenses

Alpha3 Facility Edition is a fully automated system that performs microscope configuration changes and automatic adjustments when changing objective magnification for both illumination and detection objectives.

Compatibility table for Alpha3 light sheet microscope illumination and detection objectives

Compatibility between illumination and detection objectives.

Unrivaled User Experience

Automated multi-scale microscopy systems

Alpha3 Facility Edition is a fully automated system that performs microscope configuration changes and automatic adjustments when changing objective magnification for both illumination and detection objectives.

The user can begin an imaging session with rapid sample screening using a low-magnification objective, followed by image acquisition using a higher-magnification objective on a single or multiple regions of interest.

After the user selects the desired region of interest, the system automatically selects illumination and detection objectives.

Unrivaled User Experience

3D viewer with interactive volume scan settings

The LINDA interface extension is at the heart of the user experience, generating a 3D volume preview on the fly to explore the sample before scanning. The wizard provides a live view of the current slice and “builds” a 3D rendering as the user explores the sample. LINDA allows the user to interact with the 3D rendering to set the scan volume and acquisition parameters.

3D viewer with interactive volume scan settings.

3D view of a sample with interactive volume scan settings.

Unrivaled User Experience

Imaging PaaS is a free virtual bio-imaging platform that provides on-demand services. You can access expert support for your clearing protocols, powerful computing resources for your image analysis, or advanced microscopy solutions at your convenience.

Imaging PaaS, free virtual bio-imaging platform.

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