Alphα3
Light Sheet Microscope
CUTTING-EDGE LIGHT SHEET MICROSCOPY
Alphα3
Light Sheet Microscope
CUTTING-EDGE LIGHT SHEET MICROSCOPY
Alpha3
Alpha3 is a new generation of light sheet microscope addressing the needs of high temporal resolution along with spatial high-resolution to achieve qualitative and quantitative 3D imaging of fixed or live biological specimen.
From in-vivo imaging to large cleared samples, the Alpha3 microscope delivers unprecedented image quality while keeping the necessary flexibility and modularity expected for cutting-edge scientific research instruments.
Typical applications of Alpha3 include in toto imaging of small animal models such as whole mouse embryos, morphogenesis and embryogenesis of model organisms: C.elegans, Drosophila, Zebra fish, live imaging of cell cultures, functional imaging of neuronal activity, fluorescence imaging of marine organisms or plant developmental biology.
Modular light sheet microscope
Fully flexible imaging system for easy adaptation to any experiments. Large selection of detection objectives from 2X to 60X, air, dipping lenses, clearing objectives with correction collar for RI matching. Upgreadable with new excitation and emission wavelength, illumination and detection objectives, incubation enclosure, XY tiling or fast scanning modules
High-performance imaging with smart light sheet illuminators
Multi-directional light sheet performing real time laser focus sweeping to extend the thinnest focus area over the entire field of view, artefacts-free imaging and ultra fast Z-stacking
From in-vivo to cleared samples, from small to large specimen
Macro to micro view imaging, from whole organs at sub-cellular resolution to very small specimen;
Fast and Easy sample mounting
Versatile chamber with easy access; Multiple accessories provided for sample mounting to accommodate a large variety of specimens including coverlips, glass support with clamps, molding tools; Compatible with all clearing solutions: aqueous buffers and organic solvents
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Laser source |
Laser source
Laser combiner with up to 4 or 6 laser linesExcitation wavelength from 405nm to 785nm Output power from 25 to 250mW. Fibered connection to light sheet generator |
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Light sheet generator |
Light sheet generator
Single or dual smart illuminators Multi-directional light sheet with real time focus sweeping and fast 3D scanning capabilities Minimum light sheet thickness 2µm, constant thickness across the whole field of view with focus sweeping Optimized optical unit for chromatic correction |
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Chamber & Holders |
Chamber & Holders
For sample size from µm to cm rangeChamber highly resistant to various corrosive medium, clearing agents, sea water, Standard and large chamber available, dimensions : W 21/30mm, L 70/90mm, H 25/33mm; Vol < 15ml/60ml Multiple sample mounting accessories to accommodate different sample sizes and natures: molds, coverslips, glass supports Optional: Incubation enclosure for temperature, CO2 and humidity controls. |
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Volume scanning |
Volume scanning
Z motorized stage: range 15mm, precision 0.1µm, acquisition speed 40 fpsOptional: XY motorized stages for tiling: X range 15mm, Y range 15 or 25mm, precision 0.1µm Optional: ultra fast 3D scanning module (75 images /second) |
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Detection unit |
Detection unit
Fluorescence microscope stand comprising objective turret 1 or 2 positions , eyepieces, video port, transmitted light, motorized filter wheel with up to 6 emission filters, multi-notch rejection filter
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Detection lenses |
Detection lenses
Large selection of Long Working Distance objectives with high Numerical Aperture :air, water dipping lenses, clearing objectives with correction collar RI 1.33 - 1.56 (i.e 10x0.6 and 25x0.95) Objective Magnification from 2X to 60X |
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Image Sensor |
Image Sensor
sCMOS 2048 x 2048 pixels, format 13 x 13 mm, 6.5µm square pixels., USB3 / CameraLink Interface
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Software |
Software
QtSPIM software for multi-dimensional image acquisition: Z-stacks, XY, Tiling, rotation, multi-channel and time lapse acquisition. Easy export of raw images (16 bit TIFF) with metadata, compatible with open source or commercial 3rd party software
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PC |
PC
Desktop i7-9700K 4.9Ghz – 32Go RAM - GPU GeForce GTX1060-6Go - Hard Disk 4 x 4To RAID0 - Hard Disk SSD 1To , QHD Screen 31.5”, Windows 10 / 64b Pro
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From sample mounting to image acquisition, Alpha3 performs seamless light sheet imaging. The QtSPIM software provides clear and intuitive interface to collect X,Y,Z,θ,T,λ images at maximum speed. QtSPIM controls all image acquisition parameters. Along with a workstation grade computer with an optimized configuration, the microscopy system performs ultra fast acquisition
Raw image data with their metadata are saved in 16 bits TIFF format, compatible with open source or commercial software for further 3D display and analysis
Cleared zebrafish Courtesy of Pierre Affaticati & Elodie Machado TEFOR Paris-Saclay
Plant development - Meristeme membrane Courtesy of Matthieu Ecole Normale Superieure de Lyon
Mouse Brain pegasos clearing Courtesy of University of New South Wales - Sydney
Mouse embryo
Plant development - Hypocotil microtubule
Mouse embryo iDisco clearing
Cleared Mouse Embryo, GFP staining at 10x
Cleared Zebrafish larvae head, vascular and neural staining at 20x
Astyanax
Mouse embryo
Urchins
Arabidopsis meristeme, GFP in microtubules at 40x
Mouse embryo
iDisco Cleared Mouse Embryo, motor neurones and extensions in body at 2x
Fixed Stentor, GFP cilia and DAPI poly-nuclei at 10x
In vivo Arabidopsis leaf, false colours membrane staining at 10x
Cubic 2 Mouse brain, TagRFP neurones at 20x
Fixed drosophila egg 3 channels at 20x
Cleared Mouse adipose tissue vascular network
Mouse brain - pegasos clearing
Cochlea
Human brain tissue, alzeimer patient
Mouse Cochlea Clarity
Mouse Embryo
Cleared Zebrafish EGFP vascular tag RFP cells 20x - Courtesy of Arnim Jenett & Pierre Affaticati Tefor Core Facility Gif
Arabidopsis meristeme, GFP in microtubules 40X
Fixed chicken embryo
Mouse optic chiasm
Zebrafish
Spheroids Far Red Courtesy S. Mura Institut Galien
Galleria Mellonella Larva Stage H2b - Autofluorescence Courtesy of Dr. Leyla Slamti - INRA
Fully flexible light sheet microscope - Upgreadable with new laser sources, illumination and detection objectives, incubation enclosure, XY tiling and fast scanning modules
Macro to micro view imaging: from whole organs at sub-cellular resolution to very small specimen. High-performance imaging with smart light sheet illuminators for optimal spatio-temporal resolution
From in-vivo imaging to cleared samples - Compatible with all clearing solutions: aqueous buffers and organic solvents
Multiple accessories provided to accommodate any kind of sample nature and size including coverlips, glass support with clamps, molding tools
Fast and repeatable sample mounting with easy access to chamber. 1. Clamp your sample on glass support 2. Fill-up the chamber 3. Insert sample holder 4. XYZ positioning and acquisition
Incubation enclosure for T°, CO2 and Humidity controls
QtSPIM software: intuitive interface to collect multidimensional X,Y,Z,θ,T,λ images - Raw image with metadata saved in 16 bits TIFF format for export to open source or commercial software
Alpha3 microscopy system performs seamless acquisition of TB image dataset thanks to QtSPIM software with optimized PC configuration
Smart Light Sheet Illuminators with ultrafast 3D scanning
Smart Light Sheet Illuminators with real-time focus sweeping for optimized sharpness over the entire field of view