Alpha3
Alpha3 is a new generation of light sheet microscope addressing the needs of high temporal resolution along with spatial high-resolution to achieve qualitative and quantitative 3D imaging of fixed or live biological specimen.
From in-vivo imaging to large cleared samples, the Alpha3 microscope delivers unprecedented image quality while keeping the necessary flexibility and modularity expected for cutting-edge scientific research instruments.
Typical applications of Alpha3 include in toto imaging of small animal models such as whole mouse embryos, morphogenesis and embryogenesis of model organisms: C.elegans, Drosophila, Zebra fish, live imaging of cell cultures, functional imaging of neuronal activity, fluorescence imaging of marine organisms or plant developmental biology.
Modular light sheet microscope
Fully flexible imaging system for easy adaptation to any experiments. Large selection of detection objectives from 2X to 60X, air, dipping lenses, clearing objectives with correction collar for RI matching. Upgreadable with new excitation and emission wavelength, illumination and detection objectives, incubation enclosure, XY tiling or fast scanning modules
High-performance imaging with smart light sheet illuminators
Multi-directional light sheet performing real time laser focus sweeping to extend the thinnest focus area over the entire field of view, artefacts-free imaging and ultra fast Z-stacking
From in-vivo to cleared samples, from small to large specimen
Macro to micro view imaging, from whole organs at sub-cellular resolution to very small specimen;
Fast and Easy sample mounting
Versatile chamber with easy access; Multiple accessories provided for sample mounting to accommodate a large variety of specimens including coverlips, glass support with clamps, molding tools; Compatible with all clearing solutions: aqueous buffers and organic solvents
Laser source |
Laser source
Laser combiner with up to 4 or 6 laser linesExcitation wavelength from 405nm to 785nm Output power from 25 to 250mW. Fibered connection to light sheet generator |
Light sheet generator |
Light sheet generator
Single or dual smart illuminators Multi-directional light sheet with real time focus sweeping and fast 3D scanning capabilities Minimum light sheet thickness 2µm, constant thickness across the whole field of view with focus sweeping Optimized optical unit for chromatic correction |
Chamber & Holders |
Chamber & Holders
For sample size from µm to cm rangeChamber highly resistant to various corrosive medium, clearing agents, sea water, Standard and large chamber available, dimensions : W 21/30mm, L 70/90mm, H 25/33mm; Vol < 15ml/60ml Multiple sample mounting accessories to accommodate different sample sizes and natures: molds, coverslips, glass supports Optional: Incubation enclosure for temperature, CO2 and humidity controls. |
Volume scanning |
Volume scanning
Z motorized stage: range 15mm, precision 0.1µm, acquisition speed 40 fpsOptional: XY motorized stages for tiling: X range 15mm, Y range 15 or 25mm, precision 0.1µm Optional: ultra fast 3D scanning module (75 images /second) |
Detection unit |
Detection unit
Fluorescence microscope stand comprising objective turret 1 or 2 positions , eyepieces, video port, transmitted light, motorized filter wheel with up to 6 emission filters, multi-notch rejection filter
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Detection lenses |
Detection lenses
Large selection of Long Working Distance objectives with high Numerical Aperture :air, water dipping lenses, clearing objectives with correction collar RI 1.33 - 1.56 (i.e 10x0.6 and 25x0.95) Objective Magnification from 2X to 60X |
Image Sensor |
Image Sensor
sCMOS 2048 x 2048 pixels, format 13 x 13 mm, 6.5µm square pixels., USB3 / CameraLink Interface
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Software |
Software
QtSPIM software for multi-dimensional image acquisition: Z-stacks, XY, Tiling, rotation, multi-channel and time lapse acquisition. Easy export of raw images (16 bit TIFF) with metadata, compatible with open source or commercial 3rd party software
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PC |
PC
Desktop i7-9700K 4.9Ghz – 32Go RAM - GPU GeForce GTX1060-6Go - Hard Disk 4 x 4To RAID0 - Hard Disk SSD 1To , QHD Screen 31.5”, Windows 10 / 64b Pro
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From sample mounting to image acquisition, Alpha3 performs seamless light sheet imaging. The QtSPIM software provides clear and intuitive interface to collect X,Y,Z,θ,T,λ images at maximum speed. QtSPIM controls all image acquisition parameters. Along with a workstation grade computer with an optimized configuration, the microscopy system performs ultra fast acquisition
Raw image data with their metadata are saved in 16 bits TIFF format, compatible with open source or commercial software for further 3D display and analysis