Light Sheet Microscopy Systems for Biological Imaging
PhaseView has been developing imaging technologies since 1995 and was an early developer of light-sheet microscopy systems. Over the years, we have continued to advance the technology through patented optical designs, smart illumination and high-speed 3D imaging.
Our new AlphaX Light-Sheet microscopy system.
Today PhaseView designs and manufactures light-sheet fluorescence microscopes for biological research. Our systems capture three-dimensional images of living specimens, fixed samples, cleared tissues and whole organs.
PhaseView systems are used at many research institutions, including Sorbonne Université, Université Paris-Saclay, the Hebrew University of Jerusalem, the University of Auckland, among many others.
A Configuration for Your Research
Different research areas place different demands on a light-sheet microscope. Live imaging, cleared tissues, large specimens and high-resolution imaging require different optics, sample environments and acquisition conditions.
PhaseView systems are modular. Illumination and detection objectives, lasers, detection cameras, sample chambers and holders can be selected to match the type of samples and imaging conditions used in your laboratory.
The open design keeps the sample accessible during operation. This makes it easier to integrate different mounting approaches and additional experimental equipment when needed.
PhaseView’s UltraThin technology provides high-resolution 3D imaging and is particularly valuable with 20× objectives, where resolving fine structural detail becomes especially important.
From Whole Samples to Regions of Interest
An imaging workflow can begin with an overview of the specimen. Researchers can then select regions of interest (ROIs) for acquisition at higher magnification. Multichannel imaging records different fluorescent labels, while time-lapse acquisition follows changes in living samples.
PhaseView helps laboratories select a system and configuration for their research area. The starting point is your work, not a model number or a completed technical specification.
Cleared Tissues and Whole Organs
Explore the 3D organisation of cleared tissues and whole organs. Light-sheet microscopy captures fluorescence throughout the specimen without physical sectioning. Researchers can trace labelled cells and networks, locate regions of interest and examine their position within the surrounding tissue.
PEGASOS-cleared whole mouse brain. Life-act EGFP labels actin filaments; tdTomato-tagged MARCKS labels plasma membranes. Objective: 4×, NA 0.28, WD 30 mm.
Live Imaging and Development
Follow development and cell behaviour over time. Multichannel, time-lapse imaging records a sequence of 3D volumes from a living specimen. Researchers can observe growth, cell movement and structural changes while retaining the spatial relationships between labelled structures.
Astyanax development over 28 hours. Nuclei labelled with mCherry; cytoplasm in the head labelled with GFP.
From Whole Specimen to Selected Regions
Start with a whole-specimen overview, then examine selected regions of interest (ROIs) at higher magnification. This multiscale workflow connects fine structural detail with its anatomical location. It is useful when labelled structures are sparse or distributed across a large specimen.
Multiscale zebrafish imaging: from a whole-specimen overview to selected regions and fine structural detail
A System That Can Evolve with Your Research
Research changes over time. Your microscope should be able to change with it. Compatible components can be changed or added as your laboratory explores new samples, methods and research directions.
You do not need to choose a model or prepare a detailed specification before contacting us. Tell us about your research. We can help you select a suitable system and configuration and answer any questions you may have.
PhaseView at Upcoming Conferences & Exhibitions
Meet our team at the events below. Stop by to discuss your research, ask a question or learn more about our light-sheet microscopy systems.
Neuroscience 2026
November 14–18, 2026
Walter E. Washington Convention Center, Washington, D.C.
Cell Bio 2026
December 12–15, 2026
San Diego Convention Center, San Diego, CA
Focus on Microscopy 2027
March 21–24, 2027
Osaka International Convention Center, Osaka, Japan
ELMI 2027
June 22–25, 2027
Magazzini del Cotone Congress Centre, Genoa, Italy