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3D Morphology & Anatomy with Light-Sheet Microscopy

Explore three-dimensional morphology and anatomy across intact biological specimens using light-sheet microscopy. These imaging examples reveal fine structural details at different scales, with captions describing the samples, staining and available acquisition details.

CUBIC R+ cleared whole zebrafish larva imaged at 25X — CUBIC R+ cleared whole zebrafish larva with YOYO-1 nuclear, DiD lipophilic and Alexa 647 anti-TH dopaminergic-neuron staining. Objective: Olympus XLSLPN25XGMP, 25X/1.00 NA/8 WD. Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Cleared zebrafish larva with post-synaptic and glial-cell staining — Cleared whole zebrafish larva with Alexa 488 post-synaptic staining and Alexa 555 glial-cell staining. Objective: Olympus XLPLN10XSVMP-2 MPE, 10X/0.60 NA/8 WD. Courtesy of Elodie Machado and Matthieu Simion, TEFOR Paris-Saclay.

CUBIC R+ cleared whole zebrafish larva imaged at 10X — CUBIC R+ cleared whole zebrafish larva, YOYO-1 nuclear staining, DiD lipophilic staining, Alexa 647 anti-TH dopaminergic neurons staining. Objective: Olympus XLFLUOR4X/340 (4X/0.28NA/30WD). Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

CUBIC R+ cleared whole zebrafish larva, YOYO-1 nuclear staining, DiD lipophilic staining, Alexa 647 anti-TH dopaminergic neurons staining. Objective: Olympus XLPLN10XSVMP-2 MPE (10X/0.6NA/8WD). Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Multiscale imaging of an adult zebrafish at 4X and 10X — Multiscale imaging of an adult zebrafish using 4X/0.28 NA and 10X/0.6 NA objectives. Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

ECI-transparized axolotl imaged in RFP and far-red channels — ECI-transparized axolotl imaged in two channels—RFP and far red—with a 10X/0.6 NA objective. Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Exploring 3D morphology at multiple scales?

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3D Morphology & Anatomy with Light-Sheet Microscopy

Exploring 3D morphology at multiple scales?

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