Home » Applications » Whole-Organ Imaging

Whole-Organ Light-Sheet Imaging

Explore three-dimensional views of whole organs and intact biological specimens acquired with light-sheet microscopy. These imaging examples reveal internal structures and spatial relationships, with captions describing the samples and available acquisition details.

PEGASOS-cleared whole mouse brain with Life-act EGFP and tdTomato staining — PEGASOS-cleared whole mouse brain with Life-act EGFP-labelled actin filaments and tdTomato-labelled MARCKS plasma membranes. Objective: 4X/0.28 NA/30 WD. Courtesy of Pr. Edna Hardeman and the Bio-Imaging Facility (BMIF), UNSW Sydney.

Human gastric organoids with DAPI nuclear and CDH1 Alexa 546 membrane staining — Human gastric organoids in PBS 1X with DAPI nuclear staining and CDH1/Alexa 546 membrane staining. Objective: 40X/0.80 NA/3.3 WD. Courtesy of Elena Amendola, Monte S. Angelo, University Federico II.

Multiscale imaging of a CUBIC R+ cleared whole zebrafish larva stained with YOYO-1, DiD and Alexa 647 anti-TH. Large-scale imaging: 4X illumination and detection; small-scale imaging: 10X illumination and detection. Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Multiscale imaging of an iDISCO-cleared pig brain — Multiscale 2 × 2 stitched imaging of an iDISCO-cleared pig brain at 638 nm illumination with a 690/50 nm emission filter. Imaging objectives: 4X and 10X. Courtesy of Dr. Alexander Rapp, TU Darmstadt, Germany.

Cleared mouse brain tissue with GFP-labelled CCK interneurons and Wfs1-marked pyramidal cells — CCK+ interneurons in the entorhino-hippocampal formation of the mouse brain, shown in GFP, with Wfs1-marked pyramidal cells. Objective: Olympus XLFLUOR4X/340, 4X/0.28 NA/30 WD. Courtesy of Nóra Henn-Mike, University of Pécs.

Cleared whole zebrafish brain with DAPI, Alexa 488 and DiD neuronal staining — Cleared whole zebrafish brain with DAPI, Alexa 488 neuronal and DiD neuronal staining. Objective: Olympus XLPLN10XSVMP-2 MPE, 10X/0.60 NA/8 WD. Courtesy of Isabelle Robineau and Matthieu Simion, TEFOR Paris-Saclay.

CUBIC R+ cleared whole zebrafish larva imaged at 10X — CUBIC R+ cleared whole zebrafish larva, YOYO-1 nuclear staining, DiD lipophilic staining, Alexa 647 anti-TH dopaminergic neurons staining. Objective: Olympus XLFLUOR4X/340 (4X/0.28NA/30WD). Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Imaging whole organs or large biological samples?

Tell us about your sample, clearing method and imaging requirements. We can help you identify a suitable configuration and workflow.

Whole-Organ Light-Sheet Imaging

Imaging whole organs or large biological samples?

RELATED APPLICATIONS